Protein Purification
Sigma Aldrich Fine Chemicals Biosciences Protease Inhibitor Cocktail, 1 mL
After 48 hours in medium, add fresh inhibitors in fresh medium.
Reprocell Stemolecule Y27632, 10 mg, cell-permeable small molecule, reversible ATP-competitive ROCK inhibitor, white solid, inhibits ROCK1 (220 nM), ROCK2 (300 nM), >97% purity
Stemolecule Y27632 is a cell-permeable small molecule that serves as a highly potent and selective inhibitor of Rho-associated kinase (ROCK), specifically targeting ROCK1 and ROCK2. This compound is extensively utilized in stem cell research to enhance the survival and cloning efficiency of dissociated human embryonic stem (ES) cells without compromising their self-renewal properties and pluripotency. Additionally, it improves the survival of ES cell-derived neural precursors during transplantation and significantly enhances cell recovery after cryopreservation when combined with Pifithrin-u.
- Molecular target: Rho-associated kinase (ROCK)
- Mechanism of action: Reversible ATP-competitive inhibitor
- Inhibitory concentration (Ki): ROCK1 220 nM, ROCK2 300 nM
- Purity: >97% by HPLC
- Formulation: White solid
- Solubility: Reconstitute in DMSO
- Pack size: 10 mg
CUBE BIOTECH Ultrasolute™ Amphipol 18; 1 g
1g of nanodisc copolymer Ultrasolute Amphipol 18 for the stabilization of membrane proteins outside the cell
Genscript Corporation CD96/TACTILE C110S His Hum
The receptors CD96 and TIGIT are expressed on the surface of T and natural killer (NK) cells and recent studies suggest both play important inhibitory roles in immune function CD96 has been shown to modulate immune cell activity in mice with Cd96-/- mice displaying hypersensitive NK-cell responses to immune challenge and significant tumor resistance The counterbalance between the putative inhibitory CD96 and TIGIT receptors and the activating receptor CD226 offers unique strategies for immuno-oncology drug development
Vector Laboratories Human MTHFD2 Protein Recombinant His 20ug
Human MTHFD2 Protein (Recombinant His) 20ug
Genscript Corporation MSLN/Mesothelin298-600 His
Mesothelin also known as MSLN is a protein that in humans is encoded by the MSLN gene Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF)
Genscript Corporation CD14 His Human
Human monocyte differentiation antigen CD14 is a pattern recognition receptor (PRR) that enhances innate immune responses CD14 was first identified as a marker of monocytes to signal intracellular responses upon bacterial encounters Given the absence of an intracellular tail CD14 was doubted to have the signaling capacities
Genscript Corporation EpCAM/TROP1Biotin His Avi
Epithelial Cellular Adhesion Molecule (EpCAM) also known as KS1/4 gp40 GA733-2 17-1A and TROP 1 is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF like repeats a 23 aa transmembrane segment and a 26 aa cytoplasmic domain
Abcam Plasma Membrane Protein E x traction Kit, 50 Tests
Plasma membrane protein extraction kit provides optimized buffers and reagents for the effective extraction of plasma membrane proteins from mammalian tissues and cells. The kit offers the option to either extract total cellular membrane proteins or specifically purify plasma membrane proteins. - Cited in over 200 publications - High yield and purity – consistent yield with high purity (over 90%) - Suitable for western blotting, 2-D gels, enzyme analyses, and more
The product is subject to the following: Abcam Restricted Use Statement
Zymo Research Corporation Zymo-Spin™ V-E Columns (25 Pack) w/ 15 ml Conical Reservoir
This column features a luer-lock top allowing it to be easily attached to a syringe, resesrvoir, or prefilter. The Zymo-Spin V-E features durable polypropylene construction and contains a unique silica-based matrix that allows purification of up to 125 g DNA, and 250 g RNA, in 150 l eluate. Capacity is 400 l.
Zymo Research Corporation Zymo-Spin™ III Columns (250 Pack) (Uncapped)
The Zymo-Spin III features durable polypropylene construction and contains a unique silica-based matrix that allows purification of up to 25 g DNA or RNA in 35 l eluate. Capacity is 800 l.
Zymo Research Corporation 25 ml Reservoir for Spin-Away Filters and Zymo-Spin IIICG, 25 pack
The reservoir features a durable polypropylene construction. The volume capacity of the reservoir is 25 ml.
STEMCELL Technologies RosetteSep™ Human NK Cell Enrichment Cocktail, For processing: 200 mL whole blood processed
The RosetteSep™ Human NK Cell Enrichment Cocktail is designed to isolate NK cells from whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-NK cells and red blood cells (RBCs). When centrifuged over a buoyant density medium such as RosetteSep™ DM-L (Catalog #15705) or Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified NK cells are present as a highly enriched population at the interface between the plasma and the buoyant density medium.
Bio Rad Laboratories AURUM AFFI-GEL BLUE MINI KIT
Mini kit, Affi-Gel Blue; Bio-Rad; Aurum; Consist of Micro Bio-Spin columns filled with 100 to 200 mesh Affi-Gel Blue affinity support; 25/Pk